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eppendorf real time pcr machine  (Eppendorf AG)


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    Structured Review

    Eppendorf AG eppendorf real time pcr machine
    Eppendorf Real Time Pcr Machine, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 200187 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcr+machine/Eppendorf/us12606871-589-20-24
    Average 99 stars, based on 200187 article reviews
    eppendorf real time pcr machine - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Optimization and Characterization of SHIP1 Ligands for Cellular Target Engagement and Activity in Alzheimer’s Disease Models
    Article Snippet: .. In screening mode, 10,000 cells in 20 μL/well were plated into white 96 well PCR plates (AB-3396/B, Thermo Scientific), then treated with a single concentration of compound (100 μM) or fragments (200 μM) at 37 °C for 60 min and then exposed to 3-min isothermal heating at 44.2 °C the experimentally determined Tm of HiBit-SHIP1 (n=28), and recovering to 25 °C for 3 min in a PCR machine (Eppendorf Mastercycler X50a). .. The cell plate was processed with the one step Nano-Glo ® HiBiT Lytic Detection System Protocol (Promega) and scanned with Synergy H1 plate reader (BioteK) for luminescence.

    Article Title: Maturation of GABAergic signalling times the opening of a critical period in Drosophila melanogaster
    Article Snippet: .. Collected embryos (both sexes) were transferred to 0.2 mL Eppendorf tubes and placed in a PCR machine (Eppendorf Mastercycler Gradient) programmed to maintain the ambient temperature at 25 ° C for 13 h (17:00–06:00), 32 ° C for 2 h (06:00–08:00), then held at 25 ° C thereafter. ..

    Article Title: Paeoniflorin alleviates necroptosis in acute kidney injury induced by cisplatin and lansoprazole through targeting TNFAIP3.
    Article Snippet: .. The same samples were placed in different PCR tubes and denatured for 8 min at different temperatures on a PCR machine (Eppendorf, Germany). ..

    Article Title: Xiaoyaosan alleviates CUMS rat by promoting hippocampal neurogenesis via modulating the JNK/GluR1 signaling pathway.
    Article Snippet: Objective: Depression is a syndrome of stress and emotional disorder, involving damage to synaptic plasticity in the central nervous system, particularly in the hippocampus.. Xiaoyaosan (XYS) is a classic Traditional Chinese Medicine (TCM) prescription that has shown significant antidepressant effects in both clinical and experimental settings.. This study aims to using the CUMS rat model of depression to explore the possible role of hippocampal synaptic plasticity in the pathogenesis of depression and to observe the effects of Xiaoyaosan on hippocampal synaptic plasticity mediated by the JNK/GluR1 pathway.

    Article Title: Evaluation of probiotic properties of lactic acid bacteria isolated from sheep milk and its suitability as a starter in a milk‐cereal‐based beverage
    Article Snippet: .. The DNA of the isolates was extracted and purified using a DNA isolation kit (Merc, Mumbai) and amplified in a PCR machine (Eppendorf) using the universal primers 27f (50-AGAGTTTGATCCTGGCTCAG-30) and 1492r (50- CTACGGCTACCTTGTTACGA-30) after quality checking of isolated DNA.6 The 16s rDNA sequencing of amplified DNA was performed at the “Rajiv Gandhi National Institute of Biotechnology, Hyderabad, India.” Dideoxy chain termination technique was used to determine sequencing. .. The “Basic Local Alignment Search Tool (BLAST),” available online at the “National Centre for Biotechnology Information (NCBI)” home page (http://www.ncbi.nlm.nih.gov), was used to compare homologies.

    Article Title: Synergistic and mechanistic effects of neogambogic acid in enhancing almonertinib sensitivity in non-small cell lung cancer.
    Article Snippet: .. Samples were cooled for 3 min in a PCR machine (Eppendorf, Germany). ..

    Article Title: Variation in DRD4 gene methylation in wild great tits is associated with behavioural tolerance to human disturbance but not with habitat urbanization
    Article Snippet: For PCR amplification, the 25 μl reaction mixture contained 0.625 units EpiMark Hot Start Taq DNA Polymerase (New England Biolabs, Ipswich, MA), 1x EpiMark Hot Start Taq Reaction Buffer (New England Biolabs, Ipswich, MA), 0.2 mM deoxyribonucleoside trisphosphates (dNTP), 10 μM of an unmodified forward primer and a biotin-labelled reverse primer and 20 ng bisulfite converted DNA template. .. All samples were amplified in triplicate on the same PCR machine (Eppendorf Nexus GX2). ..

    Article Title: Maturation of GABAergic signalling times the opening of a critical period in Drosophila melanogaster
    Article Snippet: .. Collected embryos (both sexes) were transferred to 0.2 mL Eppendorf tubes and placed in a PCR machine (Eppendorf Mastercycler Gradient) programmed to maintain the ambient temperature at 25 ° C for 13 h (17:00-06:00), 32 ° C for 2 h (06:00-08:00), then held at 25 ° C thereafter. ..

    Concentration Assay:

    Article Title: Optimization and Characterization of SHIP1 Ligands for Cellular Target Engagement and Activity in Alzheimer’s Disease Models
    Article Snippet: .. In screening mode, 10,000 cells in 20 μL/well were plated into white 96 well PCR plates (AB-3396/B, Thermo Scientific), then treated with a single concentration of compound (100 μM) or fragments (200 μM) at 37 °C for 60 min and then exposed to 3-min isothermal heating at 44.2 °C the experimentally determined Tm of HiBit-SHIP1 (n=28), and recovering to 25 °C for 3 min in a PCR machine (Eppendorf Mastercycler X50a). .. The cell plate was processed with the one step Nano-Glo ® HiBiT Lytic Detection System Protocol (Promega) and scanned with Synergy H1 plate reader (BioteK) for luminescence.

    RNA Extraction:

    Article Title: Xiaoyaosan alleviates CUMS rat by promoting hippocampal neurogenesis via modulating the JNK/GluR1 signaling pathway.
    Article Snippet: Objective: Depression is a syndrome of stress and emotional disorder, involving damage to synaptic plasticity in the central nervous system, particularly in the hippocampus.. Xiaoyaosan (XYS) is a classic Traditional Chinese Medicine (TCM) prescription that has shown significant antidepressant effects in both clinical and experimental settings.. This study aims to using the CUMS rat model of depression to explore the possible role of hippocampal synaptic plasticity in the pathogenesis of depression and to observe the effects of Xiaoyaosan on hippocampal synaptic plasticity mediated by the JNK/GluR1 pathway.

    cDNA Synthesis:

    Article Title: Xiaoyaosan alleviates CUMS rat by promoting hippocampal neurogenesis via modulating the JNK/GluR1 signaling pathway.
    Article Snippet: Objective: Depression is a syndrome of stress and emotional disorder, involving damage to synaptic plasticity in the central nervous system, particularly in the hippocampus.. Xiaoyaosan (XYS) is a classic Traditional Chinese Medicine (TCM) prescription that has shown significant antidepressant effects in both clinical and experimental settings.. This study aims to using the CUMS rat model of depression to explore the possible role of hippocampal synaptic plasticity in the pathogenesis of depression and to observe the effects of Xiaoyaosan on hippocampal synaptic plasticity mediated by the JNK/GluR1 pathway.

    Reverse Transcription:

    Article Title: Xiaoyaosan alleviates CUMS rat by promoting hippocampal neurogenesis via modulating the JNK/GluR1 signaling pathway.
    Article Snippet: Objective: Depression is a syndrome of stress and emotional disorder, involving damage to synaptic plasticity in the central nervous system, particularly in the hippocampus.. Xiaoyaosan (XYS) is a classic Traditional Chinese Medicine (TCM) prescription that has shown significant antidepressant effects in both clinical and experimental settings.. This study aims to using the CUMS rat model of depression to explore the possible role of hippocampal synaptic plasticity in the pathogenesis of depression and to observe the effects of Xiaoyaosan on hippocampal synaptic plasticity mediated by the JNK/GluR1 pathway.

    Purification:

    Article Title: Evaluation of probiotic properties of lactic acid bacteria isolated from sheep milk and its suitability as a starter in a milk‐cereal‐based beverage
    Article Snippet: .. The DNA of the isolates was extracted and purified using a DNA isolation kit (Merc, Mumbai) and amplified in a PCR machine (Eppendorf) using the universal primers 27f (50-AGAGTTTGATCCTGGCTCAG-30) and 1492r (50- CTACGGCTACCTTGTTACGA-30) after quality checking of isolated DNA.6 The 16s rDNA sequencing of amplified DNA was performed at the “Rajiv Gandhi National Institute of Biotechnology, Hyderabad, India.” Dideoxy chain termination technique was used to determine sequencing. .. The “Basic Local Alignment Search Tool (BLAST),” available online at the “National Centre for Biotechnology Information (NCBI)” home page (http://www.ncbi.nlm.nih.gov), was used to compare homologies.

    DNA Extraction:

    Article Title: Evaluation of probiotic properties of lactic acid bacteria isolated from sheep milk and its suitability as a starter in a milk‐cereal‐based beverage
    Article Snippet: .. The DNA of the isolates was extracted and purified using a DNA isolation kit (Merc, Mumbai) and amplified in a PCR machine (Eppendorf) using the universal primers 27f (50-AGAGTTTGATCCTGGCTCAG-30) and 1492r (50- CTACGGCTACCTTGTTACGA-30) after quality checking of isolated DNA.6 The 16s rDNA sequencing of amplified DNA was performed at the “Rajiv Gandhi National Institute of Biotechnology, Hyderabad, India.” Dideoxy chain termination technique was used to determine sequencing. .. The “Basic Local Alignment Search Tool (BLAST),” available online at the “National Centre for Biotechnology Information (NCBI)” home page (http://www.ncbi.nlm.nih.gov), was used to compare homologies.

    Amplification:

    Article Title: Evaluation of probiotic properties of lactic acid bacteria isolated from sheep milk and its suitability as a starter in a milk‐cereal‐based beverage
    Article Snippet: .. The DNA of the isolates was extracted and purified using a DNA isolation kit (Merc, Mumbai) and amplified in a PCR machine (Eppendorf) using the universal primers 27f (50-AGAGTTTGATCCTGGCTCAG-30) and 1492r (50- CTACGGCTACCTTGTTACGA-30) after quality checking of isolated DNA.6 The 16s rDNA sequencing of amplified DNA was performed at the “Rajiv Gandhi National Institute of Biotechnology, Hyderabad, India.” Dideoxy chain termination technique was used to determine sequencing. .. The “Basic Local Alignment Search Tool (BLAST),” available online at the “National Centre for Biotechnology Information (NCBI)” home page (http://www.ncbi.nlm.nih.gov), was used to compare homologies.

    Article Title: Variation in DRD4 gene methylation in wild great tits is associated with behavioural tolerance to human disturbance but not with habitat urbanization
    Article Snippet: For PCR amplification, the 25 μl reaction mixture contained 0.625 units EpiMark Hot Start Taq DNA Polymerase (New England Biolabs, Ipswich, MA), 1x EpiMark Hot Start Taq Reaction Buffer (New England Biolabs, Ipswich, MA), 0.2 mM deoxyribonucleoside trisphosphates (dNTP), 10 μM of an unmodified forward primer and a biotin-labelled reverse primer and 20 ng bisulfite converted DNA template. .. All samples were amplified in triplicate on the same PCR machine (Eppendorf Nexus GX2). ..

    Isolation:

    Article Title: Evaluation of probiotic properties of lactic acid bacteria isolated from sheep milk and its suitability as a starter in a milk‐cereal‐based beverage
    Article Snippet: .. The DNA of the isolates was extracted and purified using a DNA isolation kit (Merc, Mumbai) and amplified in a PCR machine (Eppendorf) using the universal primers 27f (50-AGAGTTTGATCCTGGCTCAG-30) and 1492r (50- CTACGGCTACCTTGTTACGA-30) after quality checking of isolated DNA.6 The 16s rDNA sequencing of amplified DNA was performed at the “Rajiv Gandhi National Institute of Biotechnology, Hyderabad, India.” Dideoxy chain termination technique was used to determine sequencing. .. The “Basic Local Alignment Search Tool (BLAST),” available online at the “National Centre for Biotechnology Information (NCBI)” home page (http://www.ncbi.nlm.nih.gov), was used to compare homologies.

    Sequencing:

    Article Title: Evaluation of probiotic properties of lactic acid bacteria isolated from sheep milk and its suitability as a starter in a milk‐cereal‐based beverage
    Article Snippet: .. The DNA of the isolates was extracted and purified using a DNA isolation kit (Merc, Mumbai) and amplified in a PCR machine (Eppendorf) using the universal primers 27f (50-AGAGTTTGATCCTGGCTCAG-30) and 1492r (50- CTACGGCTACCTTGTTACGA-30) after quality checking of isolated DNA.6 The 16s rDNA sequencing of amplified DNA was performed at the “Rajiv Gandhi National Institute of Biotechnology, Hyderabad, India.” Dideoxy chain termination technique was used to determine sequencing. .. The “Basic Local Alignment Search Tool (BLAST),” available online at the “National Centre for Biotechnology Information (NCBI)” home page (http://www.ncbi.nlm.nih.gov), was used to compare homologies.



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